Immunogenicity and antigenic relationships among spike proteins of porcine epidemic diarrhea virus subtypes G1 and G2
Identifieur interne : 001081 ( Main/Exploration ); précédent : 001080; suivant : 001082Immunogenicity and antigenic relationships among spike proteins of porcine epidemic diarrhea virus subtypes G1 and G2
Auteurs : Xiaobo Wang [République populaire de Chine] ; Jianfei Chen [République populaire de Chine] ; Da Shi [République populaire de Chine] ; Hongyan Shi [République populaire de Chine] ; Xin Zhang [République populaire de Chine] ; Jing Yuan [République populaire de Chine] ; Shibo Jiang [République populaire de Chine] ; Li Feng [République populaire de Chine]Source :
- Archives of Virology [ 0304-8608 ] ; 2015.
Abstract
Porcine epidemic diarrhea virus (PEDV) is a coronavirus that infects cells lining the small intestine of swine, resulting in vomiting, diarrhea, and dehydration. The amino acid sequence of the spike (S) protein, which is the principal target recognized by host immune cells, has multiple mutations that distinguish the two PEDV genotypes, G1 and G2. To determine whether these mutations lead to changes in antigenicity, as suggested by the failure of PEDV vaccines in China, we first optimized the codons of typical S genes of the CV777 vaccine strain (G1 subtype) and LNCT2 strain (G2 subtype) and expressed the recombinant full-length sequence of the S protein in a eukaryotic expression system. The IgG antibody levels of serum from mice immunized with purified S protein were markedly high. Antigenicity was compared by detection of polyclonal antibodies (PAbs) against the virus and S protein using an enzyme-linked immunosorbent assay (ELISA), an indirect immunofluorescence assay (IFA), and a serum cross-neutralization (SN) assay. Reactivity with the PAbs revealed significant cross-reactivity between the two PEDV subtypes, although there was a twofold difference in the antigenic responses based on PAb titers in the ELISA and IFA. Consistent with the variation in the S gene sequences, the SN titer suggested differences in the neutralization activity of the S protein between the two subtypes, which could explain the antigenic variation between the PEDV subtypes G1 and G2.
Url:
DOI: 10.1007/s00705-015-2694-6
PubMed: 26611909
PubMed Central: 7087089
Affiliations:
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Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Jianfei" sort="Chen, Jianfei" uniqKey="Chen J" first="Jianfei" last="Chen">Jianfei Chen</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410727.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 0526 1937</institution-id><institution>Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Da" sort="Shi, Da" uniqKey="Shi D" first="Da" last="Shi">Da Shi</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410727.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 0526 1937</institution-id><institution>Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Shi, Hongyan" sort="Shi, Hongyan" uniqKey="Shi H" first="Hongyan" last="Shi">Hongyan Shi</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410727.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 0526 1937</institution-id><institution>Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410727.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 0526 1937</institution-id><institution>Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Yuan, Jing" sort="Yuan, Jing" uniqKey="Yuan J" first="Jing" last="Yuan">Jing Yuan</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410727.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 0526 1937</institution-id><institution>Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.8547.e</institution-id><institution-id institution-id-type="ISNI">0000000101252443</institution-id><institution>Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Public Health Clinical Center and Shanghai Medical College,</institution><institution>Fudan University,</institution></institution-wrap>Shanghai, 200032 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shanghai</wicri:regionArea></affiliation></author><author><name sortKey="Feng, Li" sort="Feng, Li" uniqKey="Feng L" first="Li" last="Feng">Li Feng</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.410727.7</institution-id><institution-id institution-id-type="ISNI">0000 0001 0526 1937</institution-id><institution>Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,</institution><institution>Chinese Academy of Agricultural Sciences,</institution></institution-wrap>Harbin, 150001 People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Harbin</wicri:regionArea></affiliation></author></analytic><series><title level="j">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Porcine epidemic diarrhea virus (PEDV) is a coronavirus that infects cells lining the small intestine of swine, resulting in vomiting, diarrhea, and dehydration. The amino acid sequence of the spike (S) protein, which is the principal target recognized by host immune cells, has multiple mutations that distinguish the two PEDV genotypes, G1 and G2. To determine whether these mutations lead to changes in antigenicity, as suggested by the failure of PEDV vaccines in China, we first optimized the codons of typical S genes of the CV777 vaccine strain (G1 subtype) and LNCT2 strain (G2 subtype) and expressed the recombinant full-length sequence of the S protein in a eukaryotic expression system. The IgG antibody levels of serum from mice immunized with purified S protein were markedly high. Antigenicity was compared by detection of polyclonal antibodies (PAbs) against the virus and S protein using an enzyme-linked immunosorbent assay (ELISA), an indirect immunofluorescence assay (IFA), and a serum cross-neutralization (SN) assay. Reactivity with the PAbs revealed significant cross-reactivity between the two PEDV subtypes, although there was a twofold difference in the antigenic responses based on PAb titers in the ELISA and IFA. Consistent with the variation in the S gene sequences, the SN titer suggested differences in the neutralization activity of the S protein between the two subtypes, which could explain the antigenic variation between the PEDV subtypes G1 and G2.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Debouck, P" uniqKey="Debouck P">P Debouck</name></author><author><name sortKey="Pensaert, M" uniqKey="Pensaert M">M Pensaert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ducatelle, R" uniqKey="Ducatelle R">R Ducatelle</name></author><author><name sortKey="Coussement, W" uniqKey="Coussement W">W Coussement</name></author><author><name sortKey="Charlier, G" uniqKey="Charlier G">G Charlier</name></author><author><name sortKey="Debouck, P" uniqKey="Debouck P">P Debouck</name></author><author><name sortKey="Hoorens, J" uniqKey="Hoorens J">J 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populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Xiaobo" sort="Wang, Xiaobo" uniqKey="Wang X" first="Xiaobo" last="Wang">Xiaobo Wang</name></noRegion><name sortKey="Chen, Jianfei" sort="Chen, Jianfei" uniqKey="Chen J" first="Jianfei" last="Chen">Jianfei Chen</name><name sortKey="Feng, Li" sort="Feng, Li" uniqKey="Feng L" first="Li" last="Feng">Li Feng</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Shi, Da" sort="Shi, Da" uniqKey="Shi D" first="Da" last="Shi">Da Shi</name><name sortKey="Shi, Hongyan" sort="Shi, Hongyan" uniqKey="Shi H" first="Hongyan" last="Shi">Hongyan Shi</name><name sortKey="Yuan, Jing" sort="Yuan, Jing" uniqKey="Yuan J" first="Jing" last="Yuan">Jing Yuan</name><name sortKey="Zhang, Xin" sort="Zhang, Xin" uniqKey="Zhang X" first="Xin" last="Zhang">Xin Zhang</name></country></tree></affiliations></record>Pour 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